A region of interest is simply a set of labeled voxels within a shape that has a specific size and spacing. It does not contain data, only labeled voxels. However, when combined with a dataset of the same or similar shape, you can extract parameters such as the minimum, maximum, and mean values of the dataset within the volume of the ROI. With Dragonfly you can create an unlimited number of regions of interest. You can also apply an ROI to different datasets taken of the same object with different modalities or at different times to refine an ROI.
In 3D views, regions of interest can be extracted from volumes to create views containing only segmented areas, or they can be subtracted to create views of everything except the labeled voxels (see 3D Opacity).